In achieving the objectives set up in science education, it is of great importance to know the students’ opinions about laboratory practices. Hence, the purpose of the current study is to determine the students’ opinions about laboratory practices and the relationships between these opinions and variables of gender, students’ attending day-time or evening classes, grade level, and students’ effective exposure to laboratory practices during their high school education. The sampling of this study employing survey method consists of 321 first, second, third and fourth-year students attending the department of science teacher education of education faculty at Mugla University in 2010-2011 academic year. The data quantitatively collected were analyzed through independent samples t-test, one-way variance analysis, frequencies and percentages. At the end of the study, it was found that the students’ opinions do not significantly vary depending on the gender variable; yet significantly vary depending on students’ attending day-time or evening classes, grade level, and laboratory practice they were exposed during high school education.