The current study was carried out to investigate the relationship of personal characteristics of preschool pre-service teachers with some variables. The sample is 449 pre-service teachers attending to Faculty of Education, the Department of Preschool Teaching of five universities in Turkey. The data of the research was gathered through Data Collection Form and Eysenck Personality Questionnaire-Shortened Form (EPQ). In the analysis of the data, mean scores for four dimensions of EPQ were obtained. The relation between the mean scores obtained while the variables of class and gender was studied through t test and the relation between parents education level and score means was studied through “one way variance analysis (ANOVA). No relation was found between the class and parents education levels and personality sub-dimensions. A significant relation was found between the variables of gender and lying sub-dimensions, and between the mother education level and extraversion sub-dimension.