Learning is a process that takes place in our brain, in response to our experiences in formal and informal learning environments. Both of these environments have their distinctive characteristics which instigate learning process and research has also established that learning process is mediated by external and internal factors; and one such factor is learning motivation. This paper investigates learning motivation factors within formal and informal learning environments, processes and their influence upon Web-based learning (WBL), because WBL can occur in both formal and informal learning environments. To achieve this, we have discussed relevant literature on formal and informal learning environments, processes and motivational theories which have helped us to develop our research model for hypotheses testing. This research study is conducted through a survey based questionnaire, scales of which were developed on the basis of Keller’s ARCS Model, Moshinskie’s Motivation Model and Malone & Lepper’s Motivation Theory. 350 copies of the questionnaire were distributed to students at Universiti Teknologi PETRONAS. The data obtained from the survey was statistically analyzed and descriptive analysis including mean and standard deviation, reliability analysis and Pearson correlation were computed. Results showed that motivational factors associated with formal and informal learning environments and processes have strong influence upon web-based learning environments.