The aim of the study is to examine the influence of the materials generated in compliance with 5E Learning Model on students’ gains in physics laboratory applications. The research was carried out on 82 students who take the course of General Physics Laboratory II and who study their first year at the department of Science Education in Kâzım Karabekir Faculty of Education in Atatürk University within the spring semester in 2008-2009 academic year. In the study, a semi-experimental pattern with pre-test and post-test control group is used. While the applications in the experimental group was carried out using 5E Learning Model based on Structural Learning Theory, the applications in the control group was conducted using traditional verifying laboratory theory. As tools for collecting data, both before and after applications, success test in the subjects of Electrics, scientific process skill test, and physics laboratory attitude test are applied on the students in the experimental and control group. In order to test the hypothesis of the study, dependent and independent groups t-test is used. Evaluating the data gained from the pre-tests, it is determined that the groups coincide. As a conclusion of the statistic study, it is found out that 5E Learning Model applications support significantly students’ academic success, their scientific process improvement and their attitude towards physics laboratory.