This study is prepared for two basic aims: first one is to prepare a valid and reliable scale which determines laboratory selfefficacyperceptions of biology teachers and the second is to examine biology teachers’ laboratory self-efficacy perceptions from the personal characteristics points of view. The participants of this study are 126 biology teachers. In this study, to collect the data the Laboratory Self-Efficacy Scale was developed by the researcher. Frequency, arithmetic mean, t-test, factor analysis is used in evaluation of the data. At the end of the study, it was found out that laboratory self-efficacy perceptions of biology teachers are on intermediate level. In addition to this, two statically significant differences were determined between professional experience levels and laboratory self-efficacy perceptions of teachers, and betweenprofessional experience levels and laboratory self-efficacy perceptions of teachers. These differences were in favor of femaleteachers and teachers with less than 10 years experience.